Forum Category - Flux Capacitor Library preparation

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Library preparation

Forum » Flux Simulator / Library preparation

Number of threads: 5
Number of posts: 16

Discussion about reverse transcription (RT), fragmentation protocols and filtering of the library.

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Thread name	Started	Posts	Recent post
UR Fragmentation parameters	by: Joshua Dunn (guest) 05 Aug 2011 18:48	2	by gmicha 10 Aug 2011 11:46 Jump!
Why is the number of cDNA library less than the number of molecules?	by: Guorong.Xu (guest) 19 May 2010 12:00	4	by gmicha 17 Jun 2011 14:11 Jump!
Why is RPKM not proportional to number of RNA molecules? It's strange that the RPKM of generated reads for each transcript is not proportional to the number of RNA molecules of the transcript.	by: knightfeng 22 Jun 2010 05:11	3	by gmicha 23 Dec 2010 15:40 Jump!
How to get a certain number of cDNA molecules? Which parameters have an influence on the number of cDNA molecules I obtain in the library	by: Marty McFly 13 Aug 2009 09:54	2	by gmicha 13 Aug 2009 10:18 Jump!
Read distribution profiles How read distribution profiles are to be interpreted.	by: Jennifer Parker 11 Aug 2009 23:04	5	by Jennifer Parker 11 Aug 2009 23:20 Jump!